Sample
Column | Welch Ultisil LP-C18 4.6×250mm,5μm (P/N: 00208-31043) |
Detector | 240 nm |
Temperature | Room temperature |
Flow Rate | 1 ml/min |
Injection Volume | 5µl |
Mobile Phase
Mobile phase A: 0.2% phosphoric acid aqueous solution, mobile phase B: methanol
Time(min) | Mobile Phase A(%) | Mobile Phase B(%) |
0 | 80 | 20 |
20 | 60 | 40 |
30 | 60 | 40 |
31 | 80 | 20 |
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