Sample
| Column | Welch Ultisil LP-C18 4.6×250mm，5μm (P/N: 00208-31043) |
| Detector | 240 nm |
| Temperature | Room temperature |
| Flow Rate | 1 ml/min |
| Injection Volume | 5µl |
Mobile Phase
Mobile phase A: 0.2% phosphoric acid aqueous solution, mobile phase B: methanol
| Time（min） | Mobile Phase A（%） | Mobile Phase B（%） |
| 0 | 80 | 20 |
| 20 | 60 | 40 |
| 30 | 60 | 40 |
| 31 | 80 | 20 |
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