Mixed Sample:
| Column | Ultisil® F-C8, 5μm, 4.6×250mm PN: 00222-31043 |
| Mobile Phase | 0.02mol/L monopotassium phosphate pH 7.0- Methanol=65/35 |
| Detection | 288 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
| Notes | Mobile Phase pH is sensitive to resolution |
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