System adaptability
Gradient Program
| Time（min） | A% | B% |
| 0 | 60 | 40 |
| 13 | 60 | 40 |
| 27 | 20 | 80 |
| 31 | 60 | 40 |
| 35 | 60 | 40 |
| Column | Welch Ultisil Plus C18 4.6×250mm，5μm (P/N: 00260-31043) |
| Mobile Phase | A: 0.02 mol / L potassium dihydrogen phosphate, adjust pH to 3.5 with phosphoric acid; B: Methanol |
| Detector | 288nm |
| Temperature | 40℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20µl |
If you have any problem or require further information, please contact info@welchmat.com.
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