Enlarged Chromatogram(mixed solution)
| Column | Xtimate® C18, 4.6×150mm, 5μm (P/N: 00101-21041) |
| Detection | 250 nm |
| Temperature | 45℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 10 µL |
Mobile Phase
| Time(min) | Phosphate buffer (%) | Acetonitrile (%) |
| 0 | 75 | 25 |
| 20 | 75 | 25 |
| 25 | 50 | 50 |
| 35 | 50 | 50 |
| 36 | 25 | 75 |
| 45 | 25 | 75 |
If you have any problem or require further information, please contact info@welchmat.com.
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