Blank Solvent
| Column | Ultisil XB-C18 6.0×250 mm, 5 μm, |
| Mobile Phase | phosphate buffer(take 6.8g of Potassium dihydrogen phosphate, add 500ml water to dissolve it, add 5ml of triethylamine, diluted with water to 1000ml, adjust pH to 3.5 with phosphoric acid): acetonitrile = 80:20 |
| Detection | 284 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
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