Blank Solvent

ColumnUltisil XB-C18 6.0×250 mm, 5 μm,
Mobile Phasephosphate buffer(take 6.8g of Potassium dihydrogen phosphate, add 500ml water to dissolve it, add 5ml of triethylamine, diluted with water to 1000ml, adjust pH to 3.5 with phosphoric acid): acetonitrile = 80:20
Detection284 nm
Temperature30 ℃
Flow Rate1 ml/min
Injection Volume20 µl

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