
Are you still troubled by the complexity of the sample matrix? Are you still confused about how to remove pigments, oils and steroids from your samples?
Welch Materials GPC Gel Permeation Chromatography Column is faster, more efficient, and comprehensively assists your sample pretreatment process.

Specifications: 700*25mm
In the analysis of pesticide residues and semi-volatile organic compounds in various foods, agricultural products, aquatic products and traditional Chinese medicinal materials, macromolecular substances (such as pigments, oils, etc.) are generally contained in organic sample extracts. If these substances are not removed , can lead to reduced column separation efficiency, shortened inlet and column life, and thus affect data analysis results. Therefore, effective pre-purification treatment must be carried out before sample analysis of pesticide residues and semi-volatile organic compounds. However, the traditional pretreatment process takes a long time and consumes a large amount of solvent, so the large-scale application of GPC is an inevitable trend.
GPC advantage (relative to conventional SPE column)
- The filler can be reused;
- Good removal of macromolecules (pigments, oils, steroids);
- The components are not retained, and the components will not be lost.
Welch Bio-Beads S-X3 Column Features
- Wider applicability
The filler medium used is neutral, porous polystyrene divinylbenzene microspheres for size exclusion chromatography of lipophilic polymers and organic targets, with a molecular weight exclusion range of 400-14000, which can be used for separation Small molecular weight organic polymers and other hydrophobic substances, such as organochlorine, organophosphorus pesticide residues, polycyclic aromatic compounds and unsaturated lipids. The particle size of the filler is 200-400 mesh. At the same time, the stronger the aromaticity of the elution solvent, the higher the exclusion limit. The medium is compatible with reagents such as benzene, toluene, xylene, carbon tetrachloride, dimethylformamide, cyclohexane, and dichloromethane.
- Product Specifications
In addition to the common 400mm25mm specifications, Welch Materials GPC columns also launched 700mm25mm specifications. The gel permeation chromatography column introduced later is 30cm longer than the previous specification, and the previous packing process is extended, and targeted improvements have been made in the packing method for the column tube of the new specification; Welch Bio-Beads S has been added. – The amount of X3 gel filler used has better separation efficiency for the same sample.
At present, 700mm*25mm gel columns have appeared in many national inspection standards, such as: “GB23200.47-2016 National Food Safety Standard for Determination of Tetramethoxine Residues in Foods by Gas Chromatography-Mass Spectrometry”, “GB23200.93 -2016 National Food Safety Standard for Determination of Organophosphorus Pesticide Residues in Foods by Gas Chromatography-Mass Spectrometry.
Welch Bio-Beads S-X3 Column Application Case
“Determination of 5 Common Organophosphorus Pesticides in Animal Origin Foods”
GPC instrument model: Welch GPC-1600 gel chromatograph;
Gel chromatography column: Welch Bio-Beads S-X3 gel chromatography column, size 25×700mm;
Mobile phase: ethyl acetate-cyclohexane (1:1);
Flow rate: 5.0mL/min;
Injection volume: 5.0mL;
Collection time: 37-46min;
Wavelength: 254nm.



Reference standard: “GB 23200.91-2016 National Food Safety Standard for Determination of 9 Organophosphorus Pesticide Residues in Animal-derived Food-Gas Chromatography”
“Determination of Organophosphorus Pesticide Residues in Wheat”
GPC instrument model: Welch GPC-1600 gel chromatograph;
Gel chromatography column: Welch Bio-Beads S-X3 gel chromatography column, size 25×400mm;
Mobile phase: ethyl acetate-cyclohexane (1:1);
Flow rate: 5.0mL/min;
Injection volume: 5.0mL;
Collection time: 18-28min;
Wavelength: 254nm.



Reference standard: “SN/T 3768-2014 Determination method of various organophosphorus pesticide residues in export grains-Gas chromatography-mass spectrometry”
Ordering Information
P/N | Product name | Specification |
00823 -00002 | GPC Column | Welch Bio-Beads S-X3, 200-400mesh,400mm*25mm |
00823 -00003 | GPC Column | Welch Bio-Beads S-X3, 200-400mesh,700mm*25mm |
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