With the continuous development of society and the continuous popularization of nutrition knowledge, people’s health awareness is getting higher and higher, and people are more and more concerned about the relationship between fat and human health. Excessive fatty acid content in food will lead to obesity, elevated blood lipids, arteriosclerosis, and then lead to a series of cardiovascular and cerebrovascular diseases, such as hypertension and coronary heart disease. There are many common foods in life that contain fatty acids, such as fried foods such as twist, instant noodles, and fried dough sticks; baked foods such as biscuits, crackers, and shortbread; Puffed food, etc.
In order to protect human health, Welch assists in the determination of 37 kinds of fatty acids in food. WM-CN100 100m0.25mm0.2μm gas-phase capillary column is suitable for the determination of fatty acids in food according to GB 5009.168-2016 national food safety standard. It can perfectly separate 37 fatty acid methyl esters within 50 minutes, greatly shortening the analysis time and improving work efficiency . Let’s take a look at Welch’s solutions for the detection of 37 fatty acids in food.
1.Scope of application
It is suitable for the determination of 37 kinds of fatty acids in food.
Reference standard: “GB 5009.168-2016 National Food Safety Standard Determination of Fatty Acids in Food”.
2.Material
37 fatty acid methyl esters mixed standard
National standard method
Chromatographic conditions
Chromatographic column: Welch Welchrom® WM-CN100 (0.25mm*100m, 0.2μm).
Column oven: 100°C (13min) 10°C/min 180°C (6min) 1°C/min 200°C (20min) 4°C/min 230°C (10.5min);
Injector: 270°C; split ratio 30:1;
Detector: 280℃;
Column flow: 1.2mL/min;
Injection volume: 1 μL.
Spectrogram
Analysis result table
Improved method
Chromatographic conditions
Chromatographic column: Welch Welchrom® WM-CN100 (0.25mm*100m, 0.2μm).
Column oven: 0-6min: 140°C, then heated to 240°C at 4°C/min, and maintained at 240°C for 20min;
Injector: 260°C; split ratio 30:1;
Detector: 260℃;
Pre-column pressure: 36.5psi;
Injection volume: 1 μL.
Spectrogram
Analysis result table
It can be seen from the above analysis that, fully referring to the national standard detection method, Welch WM-CN100 100m0.25mm0.2μm can also complete the separation of 37 kinds of fatty acid methyl esters within 80 minutes; but the sample analysis time is too long, reducing the work efficiency. After the improvement and optimization of the detection method, this gas chromatography column can achieve baseline separation of 37 fatty acid methyl esters within 50 minutes, and the peak shape is sharp, which greatly improves the work efficiency.
In addition, Welch has been focusing on the research and development and production of gas chromatography columns for many years, which have the advantages of high inertness, low bleed, high column efficiency and long life. And has launched 22 kinds of popular capillary gas columns such as organochlorine, liquor composition analysis, TVOC analysis, etc. With excellent product performance and perfect after-sales service system, the company’s gas chromatography columns have been widely used in major universities, research institutes, pharmaceuticals , petrochemical, brewing, environmental protection and other industries.
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