The cultivation of “Immortal” HPLC column (Part 2)

It has been a while since the last article on the “Immortal” cultivation technique of the chromatographic column , and you must have a lot of smooth sailing on the road of experimental cultivation. In this issue, we I will bring you the next chapter of the column cultivation technique, to remove more “monsters and ghosts” for your future experiments.

01. Self Inspection

• Changes in frequency of use.
• Sample solution storage time.
• Rinse time, reagents, storage reagents, storage environment.
• Whether the brand and purity of the reagent have changed,
• Whether it has expired or deteriorated.
• Purchase an equivalent column and use it again.

02 Possible reasons

Reason one:
This chromatographic column has been subjected to violent shocks, such as: accidentally dropped, or rough handling during transportation (Welch Materials product packaging has undergone strict drop-resistant treatment, but there is still a small chance that the chromatographic column will be damaged by express delivery) , causing the column bed to become loose, and there was no abnormality at the beginning of use, but the column had problems after a short period of use.

Reason two:
The difference between packing batches, the production of chromatographic columns is the same as our pharmaceutical production, with strict production process (Welch Materials obtained ISO9001-2006 certification in 2015) and quality control standards, every parameter is There is a corresponding quality control range, but in the application process, there may be the possibility of strong adsorption of a specific type of substance.

Reason three:
The mobile phase and samples during use are not strictly cleaned.

Reason four:
The quality of the reagents used is questionable if the brand or batch is changed.

Reason five:
The product process has been changed, and some reagents that have irreversible damage to the chromatographic column have been introduced. For example, for chiral chromatographic columns, banned reagents are used in the sample process. Although the amount is very small, the impact on the chromatographic column is fatal.

03 Solutions

Option One
Through the above steps, it is preliminarily judged whether it is caused by the change of the use environment or the change of the chromatographic column batch.

Option two
Caused by changes in the use environment (it is usually difficult to find the exact problem point, the manufacturer can infer the cause of damage by dissecting the chromatographic column and through the performance of the chromatographic column), adjust the use environment as much as possible to be the same as before.

Option three
Due to the differences between different batches, communicate with the manufacturer to provide chromatographic columns with suitable batches of fillers, examine the tolerance of the method, and adjust the method.