Sample Solution
| Column | Ultisil Polar RP, 5μm, 4.6×250mm (P/N 00215-31043) |
| Mobile Phase | A: 1.36g KH2PO4 dissolved in 600ml water, add 0.5ml phosphate and dilute to 1000ml with water B: acetonitrile Gradient |
| Detection | 300nm |
| Temperature | 30℃ |
| Flow Rate | 1.5ml/min |
| Injection Volume | 10ul |
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