Sample solution
| Column | Welch Xtimate SEC 300 7.8x300mm, 3μm (P/N: 00237-23052) |
| Mobile Phase | 50 mM sodium dihydrogen phosphate, 50 mM disodium hydrogen phosphate, 150 mM sodium chloride dissolved in 1000 ml high purity water, adjusted to pH 7.0 with dilute sodium hydroxide solution |
| Detector | 280nm |
| Temperature | 40℃ |
| Flow Rate | 0.6ml/min |
| Injection Volume | 20ul |
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