Sample solution

ColumnWelch Xtimate SEC 300 7.8x300mm, 3μm  (P/N: 00237-23052)
  Mobile Phase50 mM sodium dihydrogen phosphate, 50 mM disodium hydrogen phosphate, 150 mM sodium chloride dissolved in 1000 ml high purity water, adjusted to pH 7.0 with dilute sodium hydroxide solution
Detector280nm
Temperature40℃
Flow Rate0.6ml/min
Injection Volume20ul

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