| Column | Ultisil® LP-CN (4.6×250 mm,5 μm) |
| Mobile Phase | Acetonitrile: 0.1mol/L ammonium acetate buffer solution (pH adjusted to 5.0 with glacial acetic acid) = 70:30 |
| Detection | 260nm |
| Temperature | 35℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
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