
Column | Ultisil® LP-C18(4.6*150mm,5μm) |
Mobile Phase | See the table below for details |
Detection | 214nm |
Temperature | 20℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 20 μL |
Mobile Phase:
Mobile Phase A: (acetonitrile)% Mobile phase B: (3.7 g NaH2PO4 H20, 0.35 g phosphoric acid aqueous solution)%
Time (min) | Mobile phase A(%) | Mobile phase B(%) |
0 | 16 | 84 |
10 | 16 | 84 |
37 | 70 | 30 |
45 | 70 | 30 |
47 | 16 | 84 |
60 | 16 | 84 |
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