ColumnUltisil® LP-C18(4.6*150mm,5μm)
Mobile PhaseSee the table below for details
Detection214nm
Temperature20℃
Flow Rate1.0 ml/min
Injection Volume20 μL

Mobile Phase:

Mobile Phase A: (acetonitrile)% Mobile phase B: (3.7 g NaH2PO4 H20, 0.35 g phosphoric acid aqueous solution)%

Time (min)Mobile phase A(%)Mobile phase B(%)
01684
101684
377030
457030
471684
601684

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