
Column | Xtimate® C8 (4.6×250 mm,5 μm) |
Mobile Phase | Acetonitrile: Buffer (4 mL triethylamine + 1300 mL water, pH adjusted to 5.3 with phosphoric acid) = 35:65 |
Detection | 250nm |
Temperature | 45℃ |
Flow Rate | 1.5 ml/min |
Injection Volume | 10 μL |
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