ColumnXtimate® C8 (4.6×250 mm,5 μm)
Mobile PhaseAcetonitrile: Buffer (4 mL triethylamine + 1300 mL water, pH adjusted to 5.3 with
phosphoric acid) = 35:65
Detection250nm
Temperature45℃
Flow Rate1.5 ml/min
Injection Volume10 μL

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