| Column | Ultisil® Diol (4.6×250 mm,5 μm) |
| Mobile Phase | N-hexane:isopropanol:10% glacial acetic acid=240:300:30 (add 0.05 mL of triethylamine to each 100 mL of mobile phase), add glacial acetic acid after mixing to adjust pH to 5.2 |
| Detection | ELSD Drift Tube Temperature: 90℃ Airflow: 3.5 bar |
| Temperature | 40℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 μL |
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