Sample Solution(full scale)
| Column | Ultisil AQ-C18, 5μm, 4.6×150mm (P/N 00207-31041) |
| Detection | 280nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
Mobile Phase
| Time/min | 0.1% formic acid | Acetonitrile |
| 0-3 | 98 | 2 |
| 3-20 | 98-80 | 2-20 |
| 20-30 | 80-60 | 20-40 |
| 30-40 | 60-20 | 40-80 |
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