Reference 2
Column | Ultisil XB-C18, 4.6×200mm, 5μm (P/N 00201-31042) |
Detection | 227nm |
Temperature | 30℃ |
Flow Rate | 1.5ml/min |
Injection Volume | 10ul |
Mobile Phase
Time/min | Acetonitrile% | Water% |
0 | 40 | 60 |
35 | 40 | 60 |
60 | 80 | 20 |
70 | 40 | 60 |
85 | 40 | 60 |
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