Thermo U3000, online derivation injection procedure, 10ul sample solution and 10ul derivatization reagent, mix well and rest for 2min before injection
| Column | Ultisil AQ-C18, 5μm, 4.6×250mm (P/N 00207-31043) |
| Mobile Phase | Methanol:Acetonitrile:Water=10:10:80 |
| Detection | 330nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
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