Broken solution:
| Column | Ultisil® LP-C8, 5μm, 4.6×250mm PN: 00209-31043 |
| Detection | 285 nm |
| Temperature | 40℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
| Time | 0.1%TFA water solution | Acetonitrile |
| 0 | 85 | 15 |
| 3 | 85 | 15 |
| 30 | 5 | 95 |
| 33 | 5 | 95 |
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