Tacrolimus on Ultisil Diol

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Reference Solution

ColumnUltisil Diol, 5μm, 4.6×250mm (P/N 00206-31043)
Mobile PhaseN-hexane:N-butyl chloride:Acetonitrile =7:2:1
Detection225nm
TemperatureAmbient
Flow Rate1.7ml/min
Injection Volume5ul

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