Sys solution and intermediate 3
| Column | Welch Ultisil® PFP（4.6×150mm，3μm) |
| Wavelength | 215 nm |
| Temperature | 40℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 10 μL |
Mobile Phase
| Time (min) | 0.1% trichloroacetic acid (%) | Methanol (%) |
| 0 | 45 | 55 |
| 20 | 45 | 55 |
| 40 | 10 | 90 |
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