Reference substance of Syringin
Gradient Program
| Time（min） | A% | B% |
| 0-20 | 10-20 | 90-80 |
| 20-30 | 20-25 | 80-75 |
| 30-40 | 40 | 60 |
| 40-50 | 10 | 90 |
| Column | Welch Ultisil XB-C18 4.6×250mm，5μm (P/N: 00201-31043) |
| Mobile Phase | A: acetonitrile B: 0.1% aqueous phosphoric acid solution |
| Detector | 220nm |
| Temperature | Room Temperature |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
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