Xtimate® C18, 4.6×250mm, 5μm (P/N: 00101-21043)
| Column | Xtimate® C18, 4.6×250mm, 5μm (P/N: 00101-21043) Ultisil® AQ-C18, 4.6×250mm, 5μm (P/N: 00207-31043) Ultisil® ALK-C18, 4.6×250mm, 5μm (P/N: 00253-31043) |
| Mobile Phase | 20 mmol/L Ammonium acetate/methanol=95/5 |
| Detection | 230 nm |
| Temperature | 40℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 10 µL |
| Notes | Column temperature has a great influence on separation, higher column temperature increases the resolution |
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