Test Sample

Gradient Program

Time(min)A%B%
06040
105545
305050
354555
366040
466040
ColumnWelch Xtimate C18 4.6x250mm,5μm  (P/N: 00101-21043)
  Mobile PhaseA: 10 mMol/L ammonium acetate + 1% tetrabutylammonium hydroxide (25% aqueous solution) pH: 6.42; B: acetonitrile
Detector238nm
Temperature40℃
Flow Rate1ml/min
Injection Volume5ul

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