ColumnUltisil® AQ-C18 4.6×250 mm, 5 μm      P/N: 00207-31043
Mobile Phase0.05mM Potassium heptane sulfonate and 0.5ml/L triethylamine, adjust pH into 3.0 with phosphoric acid: Methanol=50:50
Detection218 nm
Temperature30 ℃
Flow Rate1.0 ml/min
Injection Volume5 µl

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