Stamen Isoflavone Glucoside Test Report on Ultisil XB-C18

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Reference Solution

Gradient Program

Time(min)A%B%
02080
204060
304060
ColumnWelch Ultisil XB-C18 4.6×250mm,5μm (P/N: 00201-31043)
Mobile PhaseA: acetonitrile B: 0.2% formic acid solution;
Detector250 nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume10ul

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