Reference Solution
Gradient Program
| Time（min） | A% | B% |
| 0 | 20 | 80 |
| 20 | 40 | 60 |
| 30 | 40 | 60 |
| Column | Welch Ultisil XB-C18 4.6×250mm，5μm (P/N: 00201-31043) |
| Mobile Phase | A: acetonitrile B: 0.2% formic acid solution; |
| Detector | 250 nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
If you have any problem or require further information, please contact info@welchmat.com.
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