Reference substance
| Column | Welch Ultisil Polar-Phenyl 4.6×250, 5μm (P/N: 00252-31043) |
| Mobile Phase | 0.34% tetrabutylammonium hydrogen sulfate mixed with 0.284% anhydrous disodium hydrogen phosphate (adjust pH to 6.0 with phosphoric acid) – methanol (50:50) |
| Detector | 254nm |
| Temperature | 30℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |
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