Sodium Nitroprusside Test Report on Ultisil Pro-Phenyl

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Reference substance

ColumnWelch Ultisil  Polar-Phenyl 4.6×250, 5μm (P/N: 00252-31043)
Mobile Phase0.34% tetrabutylammonium hydrogen sulfate mixed with 0.284% anhydrous disodium hydrogen phosphate (adjust pH to 6.0 with phosphoric acid) – methanol (50:50)
Detector254nm
Temperature30℃
Flow Rate1ml/min
Injection Volume20ul

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