Sample（Polar-RP column）
| Column | Welch Ultisil Polar RP 4.6×250，5μm (P/N: 00215-31043) |
| Mobile Phase | 2g potassium dihydrogen phosphate and 0.8g tetrabutylammonium phosphate (also named tetrabutylammonium hydrogen phosphate) dissolve to 1000 mL with water, and adjust pH to 3.5 with glacial acetic acid. |
| Detector | 210nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
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