Mixed Standard
2.1 Full range
| Column | Ultisil® F-C8, 5μm, 4.6×250mm P/N: 00222-31043; Peak-front suppressor |
| Detection | 270 nm |
| Temperature | 25 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 µl |
| Notes | Sample dissolve in pure methanol, there will be a frontal peak effected by solvent |
Mobile Phase
A: 0.2% acetic acid Solution B: acetonitrile
| Time | Water% | Methanol/acetonitrile % |
| 0 | 85 | 15 |
| 15 | 65 | 35 |
| 50 | 65 | 35 |
| 55 | 40 | 60 |
| 65 | 40 | 60 |
| 66 | 85 | 15 |
| 80 | 85 | 15 |
If you have any problem or require further information, please contact info@welchmat.com.
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