Mixed Standard
2.1 Full range
Column | Ultisil® F-C8, 5μm, 4.6×250mm P/N: 00222-31043; Peak-front suppressor |
Detection | 270 nm |
Temperature | 25 ℃ |
Flow Rate | 1.0 ml/min |
Injection Volume | 20 µl |
Notes | Sample dissolve in pure methanol, there will be a frontal peak effected by solvent |
Mobile Phase
A: 0.2% acetic acid Solution B: acetonitrile
Time | Water% | Methanol/acetonitrile % |
0 | 85 | 15 |
15 | 65 | 35 |
50 | 65 | 35 |
55 | 40 | 60 |
65 | 40 | 60 |
66 | 85 | 15 |
80 | 85 | 15 |
If you have any problem or require further information, please contact info@welchmat.com.
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