Simvastatin on 4 Ultisil Columns

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Sample

Rt (min)K’Area (mAU*s)Height (mAU)Asymmetry Width (min)PlatesRSelectivity
2.20326.611213.780280.870.11582004
2.33312.184391.766730.460.113523410.671.06
2.67912.239571.622590.940.115030091.781.15
3.2751.45002e41565.436520.850.144328502.701.22
8.33033.152451.483500.560.2717520914.282.54
9.13424.150471.932560.930.1817140032.091.10
10.05915.002141.960770.980.1189396663.621.10
ColumnUltisil® XB-C18(4.6×33mm,3μm) Ultisil® AQ-C18(4.6×33mm,3μm) Ultisil® LP-C18(4.6×33mm,3μm) Ultisil® XB-C18(4.6×33mm,1.8μm)
Detection238 nm
Temperature30 ℃
Flow Rate3.0 mL/min(for Ultisil® XB-C18, 4.6×33mm, 1.8μm, flow rate is 2.0 mL/min)
Injection Volume10 µL

Mobile Phase

A: 0.1% phosphoric acid+water/acetonitrile=50/50 B: 0.1% phosphoric acid+acetonitrile

Time(min)A(%)B(%)
01000
4.51000
4.6955
82575
11.52575
11.61000
131000

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