Silymarin on Ultisil LP-C18

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Reference solution

ColumnWelch Ultisil® LP-C18(4.6×300mm,5μm))
 Detector 228nm
Temperature40℃
Flow Rate0.8 mL/min
Injection Volume10 μL
Notes\

Mobile Phase

Time0.1% Phosphoric acid(%)Methanol(%)
05545
204060
224060
22.15545
305545

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