Test sample
| Column | Welch Ultisil LP-C18 4.6×250mm，5μm (P/N: 00208-31043) |
| Mobile Phase | Methanol-water-0.5mol/L potassium dihydrogen phosphate (52.5:47.5:10) |
| Detector | 288 nm |
| Temperature | 30°C |
| Flow Rate | 0.5 ml/min |
| Injection Volume | 20µl |
| Notes | Sensitive to injection volume and dissolved sample solvent |
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