Sample
A：Thiourea
B：Phthalate
C：4-Hydroxybenzoic acid
D：2-Nitrobenzoic acid
E：2-Cyanobenzoic acid
F：2-Hydroxybenzoic acid
| Column | Welch Ultisil Polar RP 4.6×250，5μm (P/N: 00215-31043) |
| Mobile Phase | 20 mM dipotassium hydrogen phosphate(adjust pH to 2.7 with phosphoric acid) : acetonitrile = 75:25; |
| Detector | 254nm |
| Temperature | 25℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
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