ColumnUltisil® XB-C8(4.6×250mm,5μm)
Mobile Phase0.01mol/L phosphate solution: Weigh 1.56g of sodium dihydrogen phosphate,
dissolve it in 1000ml of water, add 11.3g of sulfobutyl-B-cyclodextrin sodium salt,
dissolve and mix well, adjust the pH to 3.0 with phosphoric acid, Suction
filtration, that is.
0.01mol/L phosphate solution/methanol=96/4
Detection280nm
Temperature20℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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