Separation of homoserine and 2-amino-4-butyrolactone by Xtimate C18

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ColumnXtimate® C18（4.6×250mm，5μm)
Mobile Phase0.01mol/L potassium dihydrogen phosphate (containing 2g/L sodium heptane
sulfonate, adjusted to pH 1.5 with phosphoric acid)/acetonitrile=95/5
Detection210nm
Temperature30℃
Flow Rate1.0 ml/min
Injection Volume20 μL

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