| Column | Ultisil® LP C18（4.6×250mm，5μm) |
| Mobile Phase | Phosphate buffer: Take 1.36g potassium dihydrogen phosphate, dissolve it in 1000ml water, add 2g sodium hexane sulfonate, adjust the pH value to 1.2 with phosphoric acid, pump and filter to obtain. Phosphate buffer/methanol =98/2 |
| Detection | 210nm |
| Temperature | 30℃ |
| Flow Rate | 1.2 ml/min |
| Injection Volume | 20 μL |
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