Diagram

ColumnUltisil®LP C18(4.6×250mm,5μm)
Mobile PhasePhosphate buffer: take 1.36g potassium dihydrogen phosphate, dissolve it in 1000ml water, add sodium hexanesulfonate
2g, adjust the pH to 1.2 with phosphoric acid, and filter with suction.
Phosphate buffer/methanol=98/2
Detection210nm
Temperature30℃
Flow rate1.2ml/min
Injection Volume20ul
Note\

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