| Column | Ultisil XB-C18, 5μm, 4.6×250mm PN: 00201-31043 Xtimate C18, 5μm, 4.6×250mm PN: 00101-21043 Ultisil LP-C18, 5μm, 4.6×250mm PN: 00208-31043 Ultisil Polar RP, 5μm, 4.6×250mm PN: 00215-31043 |
| Detection | 220 nm |
| Temperature | 40℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
Tetramethylammonium hydroxide solution – acetonitrile
| Time (min) | A（%） | B（%） |
| 0 | 35 | 65 |
| 21 | 57 | 43 |
| 21.01 | 35 | 65 |
| 30 | 35 | 65 |
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