Content determination of test sample

ColumnWelch Ultisil Polar RP 4.6×250,5μm (P/N: 00215-31043)
Mobile Phase0.08mol/L sodium dihydrogen phosphate solution (pH adjusted to 3.10±0.05 with phosphoric acid)-methanol=85:15
Detector276nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume20ul
NotesSensitive to injection volume and dissolved sample solvent

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