Test Sample (related substance)
| Column | Ultisil® C8, 4.6×150mm, 5μm (P/N: 00202-31041) |
| Mobile Phase | Sodium heptane sulfonate solution (2.87g sodium heptane sulfonate and 2.5g potassium dihydrogen phosphate, dissolved in water and dilute to 1000 mL, adjust pH to 3.65 with phosphoric acid solution) /acetonitrile =78/22 |
| Detection | 220 nm |
| Temperature | 30℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 20 µL |
| Notes | Sensitive to injection volume and dissolving solvent |
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