Test Sample (related substance)

ColumnUltisil® C8, 4.6×150mm, 5μm (P/N: 00202-31041)
Mobile PhaseSodium heptane sulfonate solution (2.87g sodium heptane sulfonate and 2.5g potassium dihydrogen phosphate, dissolved in water and dilute to 1000 mL, adjust pH to 3.65 with phosphoric acid solution) /acetonitrile =78/22
Detection220 nm
Flow Rate1.0 mL/min
Injection Volume20 µL
NotesSensitive to injection volume and dissolving solvent

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