Blank Solution
Mobile Phase
| Time/min | Buffer:Acetonitrile=74:26 | Water:Acetonitrile=0:70 |
| 0 | 100 | 0 |
| 50 | 100 | 0 |
| 51 | 90 | 10 |
| 80 | 90 | 10 |
| 81 | 100 | 0 |
| 100 | 100 | 0 |
| Column | Ultisil Plus C18, 4.6×150mm, 5μm (P/N 00260-31041) |
| Detection | 205nm |
| Temperature | 15℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 20ul |
| Note | Low column temperature required |
If you have any problem or require further information, please contact info@welchmat.com.
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