Mixed Imputiries

Gradient Program

Time(min)A(%)B(%)
0-301000
30-50100-600-40
50-6060-040-100
60-700100
ColumnXtimate® C18, 3.0×150 mm, 3 μm (P/N: 00101-11023)
Mobile PhaseA: 1% trifluoroacetic acid \ acetonitrile \ water =1\29\70 B: 1% trifluoroacetic acid \ acetonitrile \ water =1\75\24
Detection242 nm
Temperature40℃
Flow Rate0.75 mL/min
Injection Volume10 µL

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