Sample Solution
Gradient Program
| Time (min) | A% | B% |
| 0 | 80 | 20 |
| 15 | 40 | 60 |
| 21 | 70 | 30 |
| 30-38 | 5 | 95 |
| 39-50 | 80 | 20 |
| Column | Welch Ultisil XB-C18 4.6×250mm，5μm (P/N: 00201-31043) |
| Mobile Phase | A: 0.005M ammonium dihydrogen phosphate adjusted to pH 3.5 with phosphoric acid, B: acetonitrile |
| Detector | 215 nm |
| Temperature | 30℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 20ul |
If you have any problem or require further information, please contact info@welchmat.com.
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