Solution of impurity A
1.1 Use leading peak suppressor
Gradient Program
| Time（min） | A% | B% |
| 0 | 10 | 90 |
| 50 | 60 | 40 |
| Column | Welch Ultisil XB-C18 4.6×250mm，5μm (P/N: 00201-31043) +Leading peak suppressor |
| Mobile Phase | A: Acetonitrile B: 50mmol/L ammonium acetate solution |
| Detector | 250nm |
| Temperature | 30℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 10ul |
| Notes | Need to use leading peak suppressor because of solvent effect, Or there will be double peak on impurity A |
If you have any problem or require further information, please contact info@welchmat.com.
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