ColumnUltisil® AQ-C18 4.6×250 mm, 5 μm      P/N: 00207-31043
Mobile Phase3.16g/L ammonium formate solution (adjust pH to 7.2 with dilute ammonia water)- Acetonitrile mixed with methanol in equal proportions=37:63
Detection276 nm
Temperature40 ℃
Flow Rate1.4 ml/min
Injection Volume20 µl

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