| Column | Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043 |
| Mobile Phase | 3.16g/L ammonium formate solution (adjust pH to 7.2 with dilute ammonia water)- Acetonitrile mixed with methanol in equal proportions=37:63 |
| Detection | 276 nm |
| Temperature | 40 ℃ |
| Flow Rate | 1.4 ml/min |
| Injection Volume | 20 µl |
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