Sample
| Column | Ultisil® LP-C18, 5μm, 4.6×250mm PN: 00208-31043 |
| Mobile Phase | Water – acetonitrile – phosphate buffer (68g potassium dihydrogen phosphate, 500mL water, add 6.3mL phosphoric acid, dilute to 1000 mL with water) -0.5mol/L citrate solution -0.25mol/L sodium perchlorate solution =100:115:25:5:5 |
| Detection | 336 nm |
| Temperature | 40℃ |
| Flow Rate | 1.5 ml/min |
| Injection Volume | 20 µl |
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