Test sample（UltisilTM XB-C8 4.6×250mm，5μm）
| Column | Welch Ultisil XB-C8 4.6×250mm，5μm (P/N: 00202-31043) Welch UltisilTM LP-C8 4.6×250mm，5μm (P/N: 00209-31043) Welch Xtimate C8 4.6×250mm，5μm (P/N: 00102-21043) |
| Mobile Phase | Buffer-methanol-acetonitrile=420:300:280 |
| Detector | 254nm |
| Temperature | 40℃ |
| Flow Rate | 1ml/min |
| Injection Volume | 10ul |
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