Reference solution
1.1 Full scale
| Column | Welch Ultisil Polar RP 4.6×250mm，5μm (P/N: 00215-31043) |
| Mobile Phase | Ammonium acetate solution (25 g ammonium acetate dissolved in 1000 ml water, adjust pH to 5.0 with glacial acetic acid) /methanol =94/6 |
| Detector | 270nm |
| Temperature | 30℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20ul |
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