System suitability(related substances)
Column | Xtimate® C8, 4.6×250mm, 5μm (P/N: 00102-21043) |
Mobile Phase | Methanol/acetonitrile /0.075mol/L potassium dihydrogen phosphate solution /1.0mol/L citric acid solution =30/30/36/4 PH was adjusted to 7.0 with 10 mol/L sodium hydroxide solution |
Detection | 254 nm |
Temperature | 30℃ |
Flow Rate | 1.0 mL/min |
Injection Volume | 10 µL |
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