System suitability(related substances)

ColumnXtimate® C8, 4.6×250mm, 5μm (P/N: 00102-21043)
Mobile PhaseMethanol/acetonitrile /0.075mol/L potassium dihydrogen phosphate solution /1.0mol/L citric acid solution =30/30/36/4 PH was adjusted to 7.0 with 10 mol/L sodium hydroxide solution
Detection254 nm
Flow Rate1.0 mL/min
Injection Volume10 µL

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