System Suitability
Column | Ultisil AQ-C18, 5μm, 4.6×150mm (P/N 00207-31041) |
Detection | 220nm |
Temperature | 25℃ |
Flow Rate | 1.0ml/min |
Injection Volume | 5ul |
Mobile Phase
A: 1g/L anhydrous sodium sulfate(pH2.8); B: A/acetonitrile=95/5
Time | A | B |
0-15 | 100 | 0 |
15-25 | 100-0 | 0-100 |
25-35 | 0 | 100 |
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