| Column | Ultisil® ODS-3(4.6×150mm,5μm) |
| Mobile Phase | Please check the PDF version for details |
| Detection | 365nm |
| Temperature | 35℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 μL |
| Note | The solubility of the sample is small, and about 1 mg of ER-I should be dissolved in at least 3 mL of acetonitrile; about 1 mg of ER-II should be dissolved in at least 2 mL of acetonitrile. After being placed for two days over the weekend, some solutes were precipitated. Considering the high principal component response at this detection wavelength, a slightly lower concentration can still be accurately quantified. It is recommended to weigh about 1 mg of sample and dissolve it in 10 mL of acetonitrile to avoid quantitative errors caused by solute precipitation. |
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