ColumnUltisil® ODS-3(4.6×150mm,5μm)
Mobile PhasePlease check the PDF version for details
Flow Rate1.0 ml/min
Injection Volume10 μL
NoteThe solubility of the sample is small, and about 1 mg of ER-I should be dissolved
in at least 3 mL of acetonitrile; about 1 mg of ER-II should be dissolved in at least
2 mL of acetonitrile. After being placed for two days over the weekend, some
solutes were precipitated. Considering the high principal component response at
this detection wavelength, a slightly lower concentration can still be accurately
quantified. It is recommended to weigh about 1 mg of sample and dissolve it in 10
mL of acetonitrile to avoid quantitative errors caused by solute precipitation.

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